Dr. R. R. Pharande.

 Name                    :  Rajesh Raghunath Pharande.
Date of Birth         :  20th April 1978
Address                 :  Shiv Shakti Appt,
                             Room No 201, 2nd floor,
                             Raghunath Nagar,
                               Thane (West), Thane 400 604
Phone No.             :   9890773383
E mail                   : rpharande@gmail.com
 
EDUCATIONAL QUALIFICATION:
M. V. Sc. from MAFSU, NagpurUniversitywith 8.84 CGPA* – Year 2003
*Marks indicated are CGPA which is credit grade point average to the scale to 10.
 
PROFESSIONAL EXPERIENCE:
  • Assistant Professor, Department of Veterinary Microbiology,Bombay Veterinary College,Parel-12, Mumbai July 2009 to till date.
  • Advy Chemicals, as a Veterinary Officer, since October 2008  to July 2009
    •  ICAR, AINP on BT, Department of Microbiology, College of Veterinary and Animal Sciences, MAFSU, Parbhani as a Research Associate from November 2004 to September 2008
NATURE OF WORK DONE
 
  •  Monitor Seroprevalence of BT in Maharashtra andGoa.
    •  Collection of seroepidemiological data and analysis.
    •  Isolation and Identification of sample for BT virus.
    •  Animal Tissue culture maintenance and cell line maintenance.
    •  Field outbreak and sample collection.
    •  Record keeping and related documentation in GMP formats.
    •  Potency and toxicity determination of vaccines.
    •  Nucleic Acid Extraction. 
      •   Molecular Technical: Isolation and identification of bacteria and virus, extraction of viral RNA & DNA, PCR, RT-PCR, Electrophoresis,
Technical Skills LEARNT
RNA-PAGE.
  • Animal Tissue Culture: Cell counting using hemocytometer. Preparation of spleen cells and viability count, establishment of cell lines, MAT Assay to study cell proliferation.       
  • Cell Biology: Microscopy, Preparation of blood smears to study blood cells..
  •  Microbiology & Virology: Preparation and Isolation of pure cultures of bacteria and fungi, Gram’s staining, study of bacterial growth curve and Isolation & characterization of bacteriophages from environmental samples. Isolation of virus and propagation of virus from field samples.
  • Immunology: Precipitation & Agglutination assays, Immunoassays (ELISA, Immunoblotting). cELISA, AGPT (Agar Gel Precipitation Test), Neutralization Assays, Immunodiffusion Tests, Immunoelectrophoresis Techniques. 
Trainings Attended
 
  • Bluetongue Diseases Diagnosis by Indian Veterinary Research Institute, Izatnagar (U.P)Indiafrom February 14-23, 2005.
  • Application of Diagnostic tools for Bluetongue Disease Diagnosis by Indian Veterinary Research Institute, Izatnagar (U.P)Indiafrom February 15-21, 2007.
  • Detection of bluetongue virus group-specific antibody by indirect ELISA
using recombinant VP 7 protein by Division of Virology, IVRI, Mukteswar  August 9.-10,2007.
 
M. V. Sc Thesis 
“Studies on seroprevalence and molecular characterization of infectious bovine rhinotracheitis virus from domestic animals’’ (2003).
 
Scientific Meetings/ CONFERENCES Attended
 
  • Attended Workshop on “Recent Advances in Health and Production in
    Goats” held at Vety. College, Udgir.
  • Attended XXI Conference and National SymposiumIndiaAssociation of
     Veterinary Microbiologists, Immunologist and Specialists In Infection 
     Diseases (7-9 Feb, 2003) held at Division of Animal Health ICAR
     Research Complex for NEH Region, Meghalaya.
  • Participated in 240 hours regular programmes of NSS unit ofBombay
    VeterinaryCollege,Bombay.           
 
Publications
  • Seroprevalence and Characterization studies of infectious bovine
     rhinotracheitis virus. Oral presentation in 11th International Conferences 
     of 11th Institute for Tropical Veterinary Medicine and 16th Veterinary
     Association of Molecular Congress, held inMalaysia.
  • Seroprevalence of infectious Bovine rhinotracheitis in Marathwada region
     ofMaharashtraState. Indian J. Comp. Microbial. Immunol.Ct.
     Dis.Vol.25 No 2 (July-Dec), 115-116.
  • Study of Microbiological agents and antibiotics used in treatment of repeat
     breeder Buffaloes. Intas Polivet (2006) Vol. 7 No. 1(January –June 2006)
     Page No. 20-23.
  • Molecular analysis of infectious bovine rhinotracheitis virus. Indian. J.
     Virol Vol.18 No2 July 2007, 57-60.
  • Isolation of infectious bovine rhinotracheitis virus on MDBK cell line.  XI
     Annual Conference of IAAVR & Symposium held at Izatnagar on 27-28 
    February 2004.
  • Seroprevalence of Bluetongue virus in goat inNagpurandNasikdivision
     ofMaharashtraState. XVI Annual Convention and International
     Symposium on Management of Vector-   Borne Viruses held at ICRISAT,
     Patancheru,Hyderabad, on 7-10    February 2006.
  • A report on combined outbreak on Bluetongue and Sheep pox in
    Marathwada region ofMaharashtraState. XVI Annual Convention and
    International Symposium on Management   Of Vector-Borne Viruses held
    at ICRISAT, Patancheru,Hyderabad, on 7-10 February 2006.
  • Prevalence of Colibacillosis in Dead Poultry Chicks of Marathwada
     Region. RVJI,   Vol 4, No II: 28-30.
  • Antimicrobial resistance patterns of Salmonella and E. coli from retail
    chicken. Paper presented in 3rd convention of Indian Meat Science
Association National Symposium on Safe Meat for Good Health and Environment held at Hebbal,  Bangalore on 4-5th July 2008.HSI-23 pp 182.
  • Characteristics of Staphylococcus isolated from Rohu Fish (Lobea Rohita).
Paper  presented in 3rd convention of Indian Meat Science Association National Symposium on Safe Meat for Good Health and Environment held at Hebbal, Bangalore on 4-5th July 2008.HSI-23 pp 182.
 
Professional Memberships
• Member of Indian Associate of Veterinary Microbiologist, Immunologists
  and Specialists in Infectious Diseases.
• Member Indian Society of Virology.
• Member ofMaharashtrastate Veterinary Council 

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